DECREASE OF THE SARCOPENIA, OSTEOPENIA AND DNA DAMAGE DUE TO OXIDATIVE STRESS IN DOGS, FOR THE SUPPLY OF A FOOD SUPPLEMENT BASED ON RESVERATROL*.

The oxidative metabolism is the main responsible for the generation of waste or metabolic waste in the mitochondries, by producing more than 95% of free radical. 3 to 5% of metabolic waste are not eliminated, being this accumulation of free radicals, one of the main causes of chronic noncommunicable diseases (obesity, diabetes, cardiovascular diseases, kidney, osteoarticulars, neurodegeneratives and oncologicals). When the free radicals exceed the antioxidant capacity of the cell, the so-called oxidative stress occurs. With the objetive of reducing cell damage sold by free radicals, that are determinants for the beginning of diseases, we evaluated the treatment with Resveratrol as a food supplement. Its effects on biology were measured, with serum biomarkers of oxidative damage, to DNA, muscles and bones.

Materials and Methods

A population of 22 dogs, males and females, castrated, adults of variable ages, of indefinite race, without diseases to the clinical and biochemical examination, wormed, without the supply of any other medication or food supplements, treated in the same hygienic and feeding conditions, were selected for the study. In the day 0 (D0) the baseline clinical examination was performed with the extraction of blood for the determination of the biomarkers. During 45 days all dogs were given, 1 dose of (30 mg of Resveratrol) with each main meal, (2 diary doses in total). At the end of the treatment phase, in the day 45 (D45), a new clinical examination and extraction of blood samples was performed. From the day 45 up to the day 90 (D90), all dogs maintained the same general management conditions, but without the daily contribution of the food supplement. In the D90, new blood samples were taken for the analysis of the biomarkers. All the serums were preserved in Freezer at -80º C until the day of analysis. The biomarkers evaluated were: 8-Hydroxy-deoxy-guanosine(8(OH)dG), (ELISA method), Creatine phosphokinase (CPK) and Total Alkaline Phosphatase and osseous (FA) (spectrophotometry and enzymeimmunoassay method). The statistical analysis D0 vs D45 and D45 vs D90 was done by the student t test, for paired samples.

Results:

Biomarker Day 0 Day 45 p D 45 D 90 p 8 (OH) deoxyguanosine pg/ml 3266.7 ± 1966.1 2856.1 ± 1380.6 - 13 % Creatine phosphokinase CPK UI/L 252 ± 107.0 176.5 ± 69.4 < 0.001 176.5 ± 69.4 218.4 ± 67.9 < 0.01 Alkaline Phosphatase FA UI/L 99.3 ± 43.0 76.3 ± 27.3 < 0.001 76.3 ± 27.3 140.7 ± 93.7 < 0.001

Conclusions

The evaluated biomarkers allow us to ensure that the supply of Resveratrol as a food supplement produced: 1) decrease of the oxidative stress, 2) highly significant decrease in skeletal muscle loss (CPK), 3) highly significant decrease in bone loss (FA), 4) 13% decrease in DNA loss (8(OH)dG). The significant increase of the values of the biomarkers to the D90, demonstrates that the benefit achieved with Resveratrol at D45, is lost when it is removed from the daily diet. it is the first time that it is demonstrated that a food supplement supplied to healthy dogs. reduces the loss of muscle mass, bone mass and of DNA by oxidative stress.

Pastor, Raúl Francisco1 , Romero JE.2 , Montagna C.3 , Ferro E.4 , Corbera N.5 , Hervera M.6 , Serantes DR.7 , Ugalde J.8 * The commercial product used, was JUVENIA® DOGS – development of Microsules Argentina Laboratory. 1. Head of the Polyphenols Wine and Health Unit – Fourth Chair of Medicine – University of Buenos Aires 2. Teacher of the University of Buenos Aires, National University of La Pampa, Catholic University of Salta, Catholic University of Cordoba. 3. Consultant Microsules Argentina Laboratory. 4. Microsules Argentina Laboratory. Teacher FCV. National University of the Center of the Province of Buenos Aires 5. Research Assistant. 6. Expert Pet Nutrition, Nantes, Francia. 7. Investigator MD of the Institute of Biotechnology Research, National University of San Martín. Province. Buenos Aires, Argentina. 8. Dean of the Institute of Biotechnology Research,National University of San Martín, Province. Buenos Aires, Argentina. Contact: e-mail raulfranciscopastor@hotmail.com Telephone. +54 9 11 4411 4806