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DECREASE OF THE SARCOPENIA, OSTEOPENIA AND DNA DAMAGE DUE TO OXIDATIVE STRESS IN DOGS, THROUGH THE ADMINISTRATION OF A FOOD SUPPLEMENT BASED ON RESVERATROL*.

Metabolic waste generation in mitochondries is mainly provoked by the oxidative metabolism, since it produces more than 95% of free radical. Chronic noncommunicable diseases like obesity, diabetes, or cardiovascular diseases, among others, are caused mainly by the accumulation of these free radicals (3 to 5% of the produced metabolic waste is not eliminated, remaining in the system). 

When the free radicals exceed the antioxidant capacity of the cell, the so-called oxidative stress occurs. In order to reduce cell damage generated by free radicals, that are determinants for the beginning of diseases, we evaluated the treatment with Resveratrol as a food supplement. Its effects on biology were measured, with serum biomarkers of oxidative damage, to DNA, muscles and bones.

Materials and Methods

A group of 22 male and female dogs sharing a number of characteristics were selected for the study. All of them were neutered, adults of variable ages and indefinite race, without diseases to the clinical and biochemical examination, wormed, without any other medication or food supplements supply, treated in the same hygienic and feeding conditions. In the day 0 (D0) the baseline clinical examination was performed with the extraction of blood for biomarkers determination. During 45 days every dog was given 1 dose (30 mg) of Resveratrol in each main meal, (2 diary doses in total). At the end of the treatment phase, in the day 45 (D45), a new clinical examination and extraction of blood samples was performed. From the day 45 up to the day 90 (D90), all dogs maintained the same general management conditions, but without the daily administration of the food supplement. In the D90, new blood samples were taken with the same purpose. All the serums were preserved in Freezer at -80º C until the day of analysis. The studied biomarkers were: 8-Hydroxy-deoxy-guanosine(8(OH)dG), (ELISA method), Creatine phosphokinase (CPK) and Total Alkaline Phosphatase and osseous (FA) (spectrophotometry and enzymeimmunoassay method). The statistical analysis D0 vs D45 and D45 vs D90 was carried out by the student´s t-test, for paired samples.

Conclusions

The investigated biomarkers allow us to confirm that the administration of Resveratrol as a food supplement brought about: 1) decrease of the oxidative stress, 2) striking decline in skeletal muscle loss (CPK), 3) highly significant decrease in bone loss (FA), 4) 13% decline in DNA loss (8(OH)dG). The considerable increase of the values of the biomarkers to the D90, demonstrates that the benefit achieved with Resveratrol at D45 is diminished when removed from the daily diet. This evidence suggests for the first time ever that a food supplement administrated to healthy dogs reduces the loss of muscle and bone mass as well as of DNA caused by oxidative stress.

Pastor, Raúl Francisco1 , Romero JE.2 , Montagna C.3 , Ferro E.4 , Corbera N.5 , Hervera M.6 , Serantes DR.7 , Ugalde J.8 * The commercial product used, was JUVENIA® DOGS – development of Microsules Argentina Laboratory. 1. Head of the Polyphenols Wine and Health Unit – Fourth Chair of Medicine – University of Buenos Aires 2. Teacher of the University of Buenos Aires, National University of La Pampa, Catholic University of Salta, Catholic University of Cordoba. 3. Consultant Microsules Argentina Laboratory. 4. Microsules Argentina Laboratory. Teacher FCV. National University of the Center of the Province of Buenos Aires 5. Research Assistant. 6. Expert Pet Nutrition, Nantes, Francia. 7. Investigator MD of the Institute of Biotechnology Research, National University of San Martín. Province. Buenos Aires, Argentina. 8. Dean of the Institute of Biotechnology Research,National University of San Martín, Province. Buenos Aires, Argentina. Contact: e-mail raulfranciscopastor@hotmail.com Telephone. +54 9 11 4411 4806